To identify biomarker signatures that meet recommended accuracy thresholds for a non-sputum, biomarker-based TB triage and/or diagnostic test for children. The aims for this project is to (1) Quantify levels of known Mtb proteins using an ultra-sensitive ECL-based assay, (2) Identify host proteins, PTMs and metabolites that can serve as biomarkers for detection of childhood TB, and (3) derive and validate the diagnostic accuracy of Mtb and/or host biosignatures for childhood TB.